广西肝癌中HBV感染与N-ras基因突变的研究

应用ＰＣＲ－ＳＳＣＰ和免疫组化法检测２９例广西南部肝癌组织中的Ｎ－ｒａｓ基因突变和ＨＢＶ感染状况，结果，肝癌中Ｎ－ｒａｓ基因在第２￣３７密码子之间的突变率为７９．３％，其中２２例有２￣５个突变位点，该基因突变也见于癌旁组织，肝组织中ＨＢｓＡｇ和ＨＢｓＡｇ和ＨＢｘＡｇ检出率分别为８６．２％和７９．３％，两者具有相关性，并与Ｎ－ｒａｓ基因突变率呈相平行的趋势。因广西南部的肝癌与ＡＦＢ１污染有关，本研究

N-ras Gene Mutation and Hepatitis B Virus Infection in Guangxi Hepatocellular Carcinomas

The mutation of N-ras gene and hepatitis B virus (HBV) markers were detected in 29 cases of hepatocellular carcinomas (HCC) in Southern Guangxi by polymerase chain reaction-single strand conformation polymorphism (PCR SSCP) and immunohistochemistry.The aberrations at N-ras codons 2~37 were found at 79.31% in HCC. 2~5 mutated codons of N-ras gene were tested in 22 cases (75.86%) HCC.These mutations were also found in 80.77% liver tissue surrounding the HCCs.The HBsAg and HBxAg positive rates in liver tissue were 86.2% and 79.3% respectively.There was a positive correlation between the detection of HBsAg and HBxAg,on the other hand,there was a parallel tendency between the HBV markers and mutation rate of N-ras gene.Since the aflatoxin B1 (AFB1) contamination is a risk factor for HCC in Southern Guangxi,AFB1 may play a role the mutation of N-ras gene.