喷雾法制备载牛血清白蛋白海藻酸钙微球系统研究

采用喷雾法制备了载牛血清白蛋白(BSA)的海藻酸钙微球,通过比较微球的平均粒径和包埋率,优化了喷雾法的制备参数,使微球对BSA的包埋率大于60%、载药率达8%以上.释放液中BSA的SDS-聚丙烯酰胺凝胶电泳、Western免疫印迹以及圆二色光谱显示,该制备方法并没有对被包埋蛋白的相对分子质量、抗原性以及二级结构产生影响.微球中BSA的体外释放曲线表明该给药系统具有pH响应特性,尤其在模拟胃液中,BSA的释放速率最慢、释放量最低,可以保护蛋白类药物免受胃酸等的破坏作用,达到肠部实现控制释放.

A systematic study of preparing alginate microspheres containing BSA by spraying

The alginate microspheres containing bovine serum albumin(BSA),chosen as a model protein,was prepared by spraying.The conditions of preparation were optimized by comparing the mean diameter of particles and the encapsulation efficiency of BSA.The results indicate that the encapsulation efficiency of BSA and the BSA loading are high(60% and 8%,respectively).The process of encapsulation has no effect on relative molecular mass,the molecular structure and the antigenicity of BSA by SDS-polyacrylamide gel elect...