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稻田4种优势种蜘蛛POD同工酶的比较研究
引用本文:聂团文,孙继英,胡波,颜亨梅.稻田4种优势种蜘蛛POD同工酶的比较研究[J].湖南师范大学自然科学学报,2006,29(4):81-84.
作者姓名:聂团文  孙继英  胡波  颜亨梅
作者单位:1. 湖南师范大学医学院,中国,长沙,410006
2. 湖南师范大学生命科学学院,中国,长沙,410081
基金项目:国家自然科学基金资助项目(No.30370208)
摘    要:应用聚丙烯酰胺凝胶垂直板型电泳技术,对南方水稻田中4种优势种蜘蛛-拟环纹豹蛛(Pardosapseudoannulata)、沟渠豹蛛(Pardosa laura)、武昌獾蛛(Trochosa wuchangensis)和白跗狡蛛(Dolomedes pallitarsis)的过氧化物酶(POD)同工酶进行了比较研究.结果表明,4种蜘蛛的POD同工酶谱带清晰,不同种间差异较明显,同属于狼蛛科内3种蜘蛛的POD酶谱相似性强,与白跗狡蛛谱带差异显著,同时发现拟环纹豹蛛POD酶对农药胁迫有明显的适应性变化.

关 键 词:稻田蜘蛛  POD同工酶  聚丙烯酰胺凝胶电泳
文章编号:1000-2537(2006)04-0081-04
收稿时间:2006-01-20
修稿时间:2006年1月20日

A Comparative Study on POD Isozymes of Four Dominant Species of Spider in the Rice Field
NIE Tuan-wen,SUN Ji-ying,HU Bo,YAN Heng-mei.A Comparative Study on POD Isozymes of Four Dominant Species of Spider in the Rice Field[J].Journal of Natural Science of Hunan Normal University,2006,29(4):81-84.
Authors:NIE Tuan-wen  SUN Ji-ying  HU Bo  YAN Heng-mei
Institution:1. College of Medicine, Hunan Normal University, Changsha 410006, China; 2. College of Life Science, Hunan Normal University, Changsha 410006, China
Abstract:The pod-isoenzyme of four dominant species of spiders in the rice field in the southern China is studied using the vertical slab polyacrylamide gel electrophoresis technology.The results show that POD isoenzyme bands of four species of spiders are trenchant and there qre obvious differences in different species,POD isoenzyme bands of three species of the wolf spider has obvious comparability,but obvious differences with Dolomedes pallitarsis.It is found that there are obvious adaptation changes on pod-enzyme of the wolf spider Paedosa pseudoannulata under intimidate of the insecticide force in long time.
Keywords:spiders in the rice field  POD isoenzyme  polyacrylamide gel electrophoresis
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