| 铁皮石斛种质室温离体保存 |
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| 引用本文： | 史永忠,潘瑞炽.铁皮石斛种质室温离体保存[J].华南师范大学学报(自然科学版),1999(4):73-77. |
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| 作者姓名： | 史永忠 潘瑞炽 |
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| 作者单位： | 华南师范大学生物系，广东省植物发育生物工程重点实验室 广州，510631 |
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| 摘 要： | 铁皮石斛试管苗在１／２ＭＳ，附加０．５ｍｇ／ＬＮＡＡ、２０ｍｇ／Ｌ蔗糖、０．５ｇ／Ｌ活性炭和７ｇ／Ｌ琼脂的培养基上，在普通培养室条件下，连续保存１２个月，存在活率达１００％，培养其中添加低浓度ＮＡＡ、蔗糖和活性炭，可明显发迹保存材料的状态。
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| 关 键 词： | 铁皮石斛 种质资源 离体保存 药用植物 组织培养 |
IN VITRO CONSERVATION OF GERMPLASM AT ROOM TEMPERATURE IN DENDROBIUM OFFICINALE |
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| SHI Yongzhong, PAN Ruichi, WANG Xiaojing, YE Qingsheng, GUO Lirong.IN VITRO CONSERVATION OF GERMPLASM AT ROOM TEMPERATURE IN DENDROBIUM OFFICINALE[J].Journal of South China Normal University(Natural Science Edition),1999(4):73-77. |
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| Authors: | SHI Yongzhong PAN Ruichi WANG Xiaojing YE Qingsheng GUO Lirong |
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| Abstract: | At the temperature and irradiance of normal culture room, plantlets of Dendrobium officinalecould be conserved for 12 months without subculture. The survival rate could reach 100%. The additionof low consentraion of NAA, sucrose and activated charcoal to the medium could improve the condition ofstored plantlets. |
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| Keywords: | Dendrobium officinale Germplasm In vitro conservation |
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