| 间质胶原酶活性中心肽段的制备与表征 |
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| 引用本文： | 高磊,;蒲雪炜,;李连之,;李文智.间质胶原酶活性中心肽段的制备与表征[J].聊城师院学报,2009(3):72-74. |
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| 作者姓名： | 高磊 ;蒲雪炜 ;李连之 ;李文智 |
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| 作者单位： | [1]聊城大学化学化工学院,山东聊城252059; [2]聊城大学材料科学与工程学院,山东聊城252059 |
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| 基金项目： | 国家自然科学基金项目（No.20471025） |
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| 摘 要： | 利用Fmoc固相合成法合成了一种基质金属蛋白酶—间质胶原酶（MMP-1）活性中心的肽片段,通过RP-HPLC对粗肽进行纯化得到了高纯度的多肽,经电喷雾质谱、^1HNMR进行了表征.实验结果表明,Fmoc法合成粗肽的产率为76.0%,纯度为86.5%,经RP-HPLC纯化后纯度达到99.1%.
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| 关 键 词： | 基质金属蛋白酶 间质胶原酶 多肽 固相合成 |
Preparation and Characterization of Active Center Peptide Domain of Interstitial Collagenase |
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| Institution: | GAO Lei, PU Xue-wei, LI Lian-zhi, LI Wen-zhi (1. School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China;2. School of Materials Science and Engineering, Liaocheng University, Liaocheng 252059, China) |
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| Abstract: | The active center peptide domain of interstitial collagenase （MMP-1）,a member of matrix metalloproteinases,was synthesized by Fmoc solid phase peptide synthesis method and purified by reversed-phase high performance liquid chromatography（RP-HPLC）.The peptide was identified by ESI-MS and 1H NMR.The results show that the production rate of peptide synthesized by Fmoc strategy is 76.0% and the purity is 86.5%,while after purification on the RP-HPLC,the purity is as high as 99.1%. |
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| Keywords: | matrix metalloproteinases interstitial collagenase peptide solid-phase synthesis |
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