| 分光光度法测定藻胆蛋白含量的研究 |
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| 引用本文： | 薛志欣,;杨桂朋,;马晓梅,;张希琴.分光光度法测定藻胆蛋白含量的研究[J].烟台师范学院学报(自然科学版),2008(3):250-253. |
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| 作者姓名： | 薛志欣 ;杨桂朋 ;马晓梅 ;张希琴 |
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| 作者单位： | [1]青岛大学化学化工与环境学院,山东青岛266071; [2]中国海洋大学海洋化学理论与工程技术实验室,山东青岛266003 |
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| 基金项目： | 山东省“泰山学者”建设工程专项经费资助课题（JS200510016） |
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| 摘 要： | 从多管藻和螺旋藻中提取了藻胆蛋白，利用藻胆蛋自在可见光区具有吸收的特点，建立了藻胆蛋白含量的快速测定方法．在波长565和620nm处测定藻红蛋白和藻蓝蛋白在0-1．2mg／mL内具有良好的线性关系，r藻红=0．9995，r藻蓝=0．9998，该方法测定藻红、藻蓝蛋白的回收率分别为99．07％和99．11％．与传统的Lowry法相比，该方法测定蛋向质简便快速．重现性好．
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| 关 键 词： | 藻胆蛋白 分光光度法 蛋白质含量测定 |
Study on Extraction of Phycobiliproteins and Their Content Determination Based on Spectrophotometry |
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| Institution: | XUE Zhi-xin,YANG Gui-peng,MA Xiao-mei,ZHANG Xi-qin( 1. College of Chemical Engineering,Qingdao University ,Qingdao 266071 ,China;2. Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education, Ocean University of China, Qingdao 266003, China) |
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| Abstract: | The phycoerythrin is extracted from Polysiphonia urceolata and Spirulina platensis. A simple and rapid method for the determination of phycoerythrin content is established＂ based on the adsorption of R-phycoerythrin（ R-PE ） and C-phycocyanin （C-PC） under visible light area. The absorbance value at 565 nm and 620 nm had a good linearity with the mass concentration of R-PE and C-PC in the range 0-1.2 mg/mL, with rR-PE =0. 9995 and rC-PC =0. 9998. The recovery ratio for R-PE and C-PC is 99. 07% and 99. 11% ,respectively. This determination method has a good reproducibility, which is simpler and rapider than the conditional detection-Lowry method. |
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| Keywords: | phycobiliprotein spectrophotometry protein content determination |
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