酸奶发酵剂高浓度培养的研究

对传统酸奶菌保加利亚乳杆菌和嗜热链球菌进行了高浓度混合培养的研究。综合利用中和法、补料培养法及好气培养法等，确定了Ｌ．ｂ和Ｓ．ｔ的最佳培养工艺条件。其最佳培养基配比为：脱脂奶粉１０％、乳清粉１％、酵母膏１％、磷酸氢二铵０．２５％；培养过程控制ｐＨ值为５．８～６．３，摇床培养（（８５～１００）ｒ／ｍｉｎ）４ｈ后，补加２％乳糖；继续培养至５．５ｈ后结束，菌数达到２５．７５亿个／ｍＬ

THE STUDY ON THE CULTURE WITH HIGH CELLS POPULATION ON YOGHURT STARTER

The mixed culture with high cells population of L.b and S.t was studied.Neutralizer and buffer salft was used and the methods of feed batch culture and aerobiotic culture were adopted.The optimal culturing conditions were got.The optimal medium contained the following ingredients:10% NFMS,1% whey powder,1% yeast extract and 0.25% ammonium phosphate dibasic;The pH was controlled at an interval of 5.86.3,shaking culture with rotating speed (85100) r/min was used,after 4 hours culture,2% lactose was added to the medium and the cells population got to 2.575109 cfu/ml after 5.5 hours culture.