| 固定化果糖基转移酶的理化性质及稳定性研究 |
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| 引用本文： | 魏远安,姚评佳,谢庆武,梁锦添. 固定化果糖基转移酶的理化性质及稳定性研究[J]. 广西大学学报(自然科学版), 2002, 27(1): 1-4 |
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| 作者姓名： | 魏远安 姚评佳 谢庆武 梁锦添 |
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| 作者单位： | 广西大学,生物技术试验中心,广西,南宁,530004 |
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| 基金项目： | 国家自然科学基金 (2 9772 0 0 6) |
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| 摘 要： | 果糖基转移酶（FTS）能有效地把蔗糖转化为蔗果低聚糖，我们比较研究了从诱变的米曲霉GX0010菌株提取的果糖基转移酶（FTS）和固定化果糖基转移酶（IFTS）的理化性质及其稳定性，结果表明：两者的最适PH为5.5,IFTS比FTS的适宜温度范围扩宽了，而且IFTS的酸碱稳定性，热稳定性和贮存稳定性明显提高，间歇式生产时IFTS的使用半衰期为54次。
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| 关 键 词： | 固定化果糖基转移酶 理化性质 稳定性 蔗果低聚糖 蔗糖 酶活力 PH值 温度 |
| 文章编号： | 1001-7445(2002)01-0001-04 |
| 修稿时间： | 2002-01-10 |
Study on characteristics and thermostability of immobilized fructosyltransferase |
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| WEI Yuan an,YAO Ping jia,XIE Qing wu,LIANG Jin tian. Study on characteristics and thermostability of immobilized fructosyltransferase[J]. Journal of Guangxi University(Natural Science Edition), 2002, 27(1): 1-4 |
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| Authors: | WEI Yuan an YAO Ping jia XIE Qing wu LIANG Jin tian |
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| Abstract: | Fructosyltransferase (FTS; EC 2.4.1.9) converts sucrose effectively to fructoolingosaccharide (FOS).The catalytic properties of immobilized fructosyltransferase (IFTS) were investigated and compared with those of extracted fructosyltransferase from mutagenesed Aspergillus oryzae GX0010.It was found that both free and immobilized FTase have the optimum pH of 5.5. But the Ph stability and the thermal stability and bin stability of IFTS increased substantially.The halflife of IFTS is 54 times in batch operation. |
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| Keywords: | fructosyltransferase(FTS) immobilized fructosyltransferase (IFTS) fructoolingosaccharide (FOS) |
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