Configuration of nucleolar DNAin situ in nucleolus ofAllium cepa cells |
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Authors: | Wei Tao Jie He Mingda Jiao Mengyuan He Shui Hao |
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Affiliation: | Department of Cell Biology, College of Life Sciences, Peking University, Beijing 100871 ,China;(2)Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024 ,China |
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Abstract: | The location and configuration of nucleolar DNA have not been determined for a long time. In this paper, we have observed the nucleolar ultrastructure and the character of nucleolar DNA in Allium cepa cells by conventional electron microscopy and the cytochemical NAMA-Ur DNA specific staining method. Furthermore, we have properly improved the NAMA-Ur method so as to analyze the location and configuration of nucleolar DNA in situ. Our results indicated that the nucleolar DNA in Allium cepa cells is mainly located at the border between fibrillar centers and dense fibrillar component, especially distributed in the configuration of encircling the fibrillar centers. |
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Keywords: | nucleolus NAMA-Ur method configuration of nucleolar DNA Allium cepa |
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