| 从啤酒废酵母中提取谷胱甘肽的条件优化 |
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| 引用本文： | 常秀莲,郑秀芬,刘建明,王文华.从啤酒废酵母中提取谷胱甘肽的条件优化[J].烟台大学学报(自然科学与工程版),2009,22(3):189-192. |
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| 作者姓名： | 常秀莲 郑秀芬 刘建明 王文华 |
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| 作者单位： | 1. 烟台大学,海洋学院,山东,烟台,264005
2. 烟台市环境监测中心,山东,烟台,264000 |
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| 基金项目： | 山东省科技攻关项目，烟台大学博士基金 |
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| 摘 要： | 采用热水抽提法，综合考察提取条件对谷胱甘肽和杂质蛋白质溶出率的影响，对从啤酒废酵母中提取谷胱甘肽的工艺条件进行了优化．研究了pH、温度、时间和料水比对产品谷胱甘肽和杂质蛋白质提取量的影响，进行了单因素和正交实验设计，得到了最佳的热水抽提条件：温度70℃，pH2．25，时间15min，料水比1：4．
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| 关 键 词： | 啤酒废酵母 谷胱甘肽 蛋白质 热水提取 |
Optimization of Conditions for Extractiong Glutathione from Abandoned Beer Yeasts |
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| CHANG Xiu-lian,ZHENG Xiu-fen,LIU Jian-ming,WANG Wen-hua.Optimization of Conditions for Extractiong Glutathione from Abandoned Beer Yeasts[J].Journal of Yantai University(Natural Science and Engineering edirion),2009,22(3):189-192. |
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| Authors: | CHANG Xiu-lian ZHENG Xiu-fen LIU Jian-ming WANG Wen-hua |
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| Institution: | CHANG Xiu-lian, ZHENG Xiu-fen, LIU Jian-ming , WANG Wen-hua ( 1. School of Ocean, Yantai University, Yantai 264005, China; 2. Environmental Monitoring Central Station of Yantai, Yantai 264000, China) |
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| Abstract: | Based on the integration of maximizing the high product yield and minimizing the low dissolved protein concentration, glutathione is extracted and optimized by hot water from abandoned beer yeasts. The effects of pH, temperature, extraction time and the ratio of yeasts to water on the extraction amounts of glutathione and protein have been investigated by single factor and orthogonal tests. The results show that the optimal extracting conditions are as follows ： extraction temperature 70 ℃, pH value 2.25, extraction time 15 min, and the ratio of yeasts to water 1：4. |
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| Keywords: | abandoned beer yeasts glutathione protein hot water extraction |
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