| 一种新型细胞趋化迁移微流控通道的构建及表征 |
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| 引用本文： | 肖成茂,蔡绍皙,蒋稼欢,晏小清.一种新型细胞趋化迁移微流控通道的构建及表征[J].科学通报,2011,56(10):751-755. |
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| 作者姓名： | 肖成茂 蔡绍皙 蒋稼欢 晏小清 |
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| 作者单位： | 生物流变科学与技术教育部重点实验室;重庆大学生物工程学院; |
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| 基金项目： | 国家自然科学基金(10872224,2010011515和30870607); 中央高校基本科研业务费(CDJXS10231115); 青年科学基金(10902130)资助 |
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| 摘 要： | 细胞迁移、趋化在多种生理和病理过程中扮演着重要角色. 传统方法, 如琼脂糖平板法、Transwell 小室法等大多是在单因素条件下检测细胞迁移情况, 不能建立起多参数可控的实验环境. 微粒流控芯片能保证迁移试验在多参数条件下完成并进行实时观测, 然而制作微芯片采用的光刻法工艺复杂、成本昂贵. 本文采用聚二甲基硅氧烷(PDMS)铸模, 抽去微丝后制成一种高500 μm 的微通道, 在通道中可生成持续的较恒定的浓度梯度和压力梯度. 将通道应用于细胞趋化试验中, 进一步验证了这种微流动通道制作技术的可行性和适用性.
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| 关 键 词： | 趋化 微流控 十字通道 浓度梯度 压力梯度 |
| 收稿时间： | 2011-01-11 |
A new micro-fluidic channel that provides stable biochemical gradients for cell migration and chemotaxis |
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| XIAO ChengMao,CAI ShaoXi,JIANG JiaHuan, & YAN XiaoQing, Key Laboratory of Biorheological Science , Technology,Ministry of Education,Chongqing ,China, Bioengineering College,Chongqing University.A new micro-fluidic channel that provides stable biochemical gradients for cell migration and chemotaxis[J].Chinese Science Bulletin,2011,56(10):751-755. |
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| Authors: | XIAO ChengMao CAI ShaoXi JIANG JiaHuan & YAN XiaoQing Key Laboratory of Biorheological Science Technology Ministry of Education Chongqing China Bioengineering College Chongqing University |
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| Institution: | XIAO ChengMao1,2,CAI ShaoXi1,2*,JIANG JiaHuan1,2 & YAN XiaoQing1,2 1 Key Laboratory of Biorheological Science and Technology,Ministry of Education,Chongqing 400044,China,2 Bioengineering College,Chongqing University |
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| Abstract: | Cell migration and chemotaxis play an important role in many physiological and pathological processes. Traditional methods such as agarose plate culture and the transwell chamber model can only detect cell migration induced by a single factor. Microfluidic chips overcome this limitation and further provide multi-parameter conditions and real-time observation. However, microchips are mostly constructed using photolithography, a typically expensive procedure. We introduce an accessible platform made of polydi... |
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| Keywords: | chemotactic migration cross-channel concentration gradient pressure gradient |
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