| 基于纳米金表面竞争杂交反应的新型DNA检测方法(英文) |
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| 引用本文： | 杨帆,张杨刚,田橙. 基于纳米金表面竞争杂交反应的新型DNA检测方法(英文)[J]. 中国科学技术大学学报, 2011, 0(11) |
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| 作者姓名： | 杨帆 张杨刚 田橙 |
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| 作者单位： | 中国科学技术大学化学系;上海移宇科技有限公司; |
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| 摘 要： | 基于纳米金表面DNA的竞争杂交反应和纳米金粒子的离心分离-富集过程,建立了一种DNA荧光检测方法,实现对飞摩尔级目标DNA的高灵敏度检测.同时通过DNA的竞争杂交过程并结合缓冲液离子强度的阶跃式升高,实现了对目标DNA上单个碱基错配的有效分辨.本研究还提供了一种简单有效的DNA分离-富集方法,相对于通常使用的磁珠分离过程更为经济方便.
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| 关 键 词： | 纳米金 DNA检测 单碱基错配 DNA富集 可视化检测 |
Agold nanoparticle based competitive hybridization/centrifugation assay for fluorescent detection of femtomole DNA with enhanced SNP discriminability |
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| YANG Fan,,ZHANG Yanggang,TIAN Cheng. Agold nanoparticle based competitive hybridization/centrifugation assay for fluorescent detection of femtomole DNA with enhanced SNP discriminability[J]. Journal of University of Science and Technology of China, 2011, 0(11) |
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| Authors: | YANG Fan ZHANG Yanggang TIAN Cheng |
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| Affiliation: | YANG Fan1,2,ZHANG Yanggang1,TIAN Cheng1(1.Department of Chemistry,University of Science and Technology of China,Hefei 230026,China,2.Shanghai Medtrum technology Co.,Ltd.,Shanghai 200000,China) |
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| Abstract: | A sensitive DNA detection assay based on fluorescence spectrometry was developed.The assay incorporates gold nanoparticle based probe separation and competitive DNA hybridization to enhance Femtomole sequence-specific DNA detection.Through the stepped-increase process of the ionic strength in buffer solution,single-base mismatch resolvability was achieved.A simple and economical way for DNA target enrichment from sample solutions is also given. |
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| Keywords: | gold nanoparticle target DNA detection single-base mismatch resolvability DNA target enrichment |
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