Comparison of protein synthesis profiles in chronic lymphocytic leukaemia cells and B-lymphocytes from peripheral blood,cord blood and tonsil |
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| Authors: | F K Saunders J Lawry D A Winfield J R Goepel B W Hancock R M Sharrard M H Goyns |
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| Institution: | (1) Institute for Cancer Studies, University Medical School, Beech Hill Road, S10 2RX Sheffield;(2) Department of Haematology, Royal Hallamshire Hospital, S10 2JF Sheffield;(3) Department of Pathology, University Medical School, S10 2RX Sheffield, (UK);(4) Present address: Department of Biochemistry, University of Leicester, Biological Sciences Building, University Road, LE1 9HN Leicester, (UK) |
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| Abstract: | 2D-gel electrophoresis was used to investigate protein synthesis in leukaemic cells from a series of 15 chronic lymphocytic leukaemia (CLL) patients, and in non-malignant B-cell populations from different sources. The protein synthesis profiles of CD5+ B-cells from umbilical cord blood and from tonsil were determined, and the levels of expression of their proteins were observed to be similar to the CLL cells. The CD5-cells from cord blood resembled peripheral blood B-lymphocytes, and the protein synthesis profile of CD5-cells from tonsils was very complex. One protein was also identified which consistently appeared to be synthesised at a low level in CD5+ B-cells from tonsil but which was always more prominant in CLL cells and other non-malignant B-lymphocytes. On the basis of these data it is possible that the closest non-malignant counterpart to CLL is the CD5+ B-lymphocyte from cord blood. |
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| Keywords: | CLL 2D-gel protein CD5+ve B-lymphocyte |
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