| 错配PCR对牛X染色体相关基因杂合子的检测 |
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| 引用本文： | 曹更生,王毅彰,周文平.错配PCR对牛X染色体相关基因杂合子的检测[J].河南大学学报(自然科学版),2009,39(4). |
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| 作者姓名： | 曹更生 王毅彰 周文平 |
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| 作者单位： | 1. 河南大学,生命科学学院,河南,开封,475004
2. 安阳师范学院,资源环境与旅游学院,河南,安阳,455002 |
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| 基金项目： | 国家自然科学基金，转基因生物新品种培育重大专项课题"高品质转基因奶牛新品种培育"(2008ZX08007-001)资金联合资助 |
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| 摘 要： | 利用DNA测序技术,在荷斯坦奶牛胎儿中找到X染色体基因GLRA2 3'端的杂合位点(C/T),设计错配引物在该位点对胎儿及其父本、母本基因组进行PCR扩增,建立起GLRA2基因的错配PCR技术,该技术可以快速在群体基因组中检测这一杂合位点.
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| 关 键 词： | 错配PCR 杂合子 单核苷酸多态 |
Detection of X-linked Heterozygous Gene in Cow with Mismatch PCR |
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| CAO Geng-sheng,WANG Yi-zhang,ZHOU Wen-ping.Detection of X-linked Heterozygous Gene in Cow with Mismatch PCR[J].Journal of Henan University(Natural Science),2009,39(4). |
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| Authors: | CAO Geng-sheng WANG Yi-zhang ZHOU Wen-ping |
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| Institution: | 1.College of Life Science;Henan University;Henan Kaifeng 475004;China;2.School of Environment Resource and Tourism;Anyang Normal University;Henan Anyang 455002;China |
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| Abstract: | Using DNA sequencing,a heterozygous locus(C/T) was identified at the 3' region of GLRA2 gene,a X-linked housekeeping gene in cow.To further validate this SNP(single-nucleotide polymorphism) locus,the technique of mismatch PCR was established and amplified to examine the samples of embryo,paternal and maternal genomic DNA.The result suggests that this technique can also be applied to verify the same locus in the cow population. |
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| Keywords: | mismatch PCR heterozygous gene single-nucleotide polymorphism |
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