| 产辅酶Q10大肠杆菌工程菌的构建 |
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| 引用本文： | 李家洲,谢明权,李安兴,罗晓春. 产辅酶Q10大肠杆菌工程菌的构建[J]. 华南理工大学学报(自然科学版), 2012, 40(5): 90-95 |
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| 作者姓名： | 李家洲 谢明权 李安兴 罗晓春 |
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| 作者单位： | 1. 华南理工大学生物科学与工程学院,广东广州,510006
2. 中山大学水生经济动物研究所,广东广州,510275 |
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| 基金项目： | 广东省自然科学基金资助项目，广东省工业攻关项目 |
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| 摘 要： | 克隆放射型根瘤菌ddsA基因,用于构建red重组的打靶片段,通过同源重组替换大肠杆菌基因组上的ispB基因,使合成辅酶Q8的大肠杆菌具备合成辅酶Q10的能力.通过强化辅酶Q合成过程中的ddsA、ubiA、ispA、idi等关键酶基因,可使大肠杆菌辅酶Q10的合成能力提高126.9%.综合分析表明,ddsA与ubiA为辅酶Q10合成的关键基因,ispA与idi为辅酶Q10合成的次关键基因.
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| 关 键 词： | 辅酶Q 大肠杆菌 ddsA基因 工程菌 同源重组 |
Construction of Engineering Escherichia coli for Production of Coenzyme Q10 |
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| Li Jia-zhou , Xie Ming-quan , Li An-xing , Luo Xiao-chun. Construction of Engineering Escherichia coli for Production of Coenzyme Q10[J]. Journal of South China University of Technology(Natural Science Edition), 2012, 40(5): 90-95 |
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| Authors: | Li Jia-zhou Xie Ming-quan Li An-xing Luo Xiao-chun |
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| Affiliation: | 1(1.School of Biological Science and Engineering,South China University of Technology,Guangzhou 510006,Guangdong,China; 2.Institute of Aquatic Economic Animals,Sun Yat-Sen University,Guangzhou 510275,Guangdong,China) |
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| Abstract: | In this paper,decaprenyl diphosphate synthase(ddsA) gene from Rhizobium radiobacter was cloned and used to construct a target fragment for red recombination.Then,the ispB gene in Escherichia coli genome was substituted with ddsA gene by homologic recombination,which made the recombinant Escherichia coli be capable of synthesizing CoQ10 instead of CoQ8.Moreover,some key enzyme genes for CoQ synthesis,such as ddsA,ubiA,ispA and idi,were strengthened to improve the production of CoQ10(up to 126.9%).Comprehensive analyses show that ddsA and ubiA are key genes,while ispA and idi are secondary key genes for CoQ10 synthesis. |
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| Keywords: | coenzyme Q Escherichia coli ddsA gene engineering bacteria homologous recombination |
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