高糖高胆固醇诱导EA.hy926细胞凋亡中对HSPD1基因表达的影响

用高糖（25mmol/L葡萄糖）和胆固醇（0.8, 1.6, 3.2mmol/L）处理EA.分析高糖高胆固醇诱导EA.hy926细胞凋亡中对HSPD1基因表达的影响，研究表明随胆固醇浓度的增加，EA.hy926细胞活性下降，呈浓度依赖性.0.8mmol/L胆固醇低糖培养液培养12h的细胞活性与正常对照组无显著差异（P>0.05），而0.8mmol/L胆固醇高糖培养液培养12h的细胞活性与正常对照组（高糖培养液+0mmol/L胆固醇）具显著性差异(P<0.01).低糖或高糖培养液+0.8mmol/L胆固醇处理EA.hy926细胞不同时间后，细胞活性呈时间依赖性下调，48h后细胞大部分变圆，脱落.高糖高胆固醇处理的细胞大部分发生凋亡（50%），HSPD1基因表达显著上调.

The HSPD1 Expression in EA.hy926 Cell Apoptosis Induced by Hight Glucose and Hight Cholesterol

To explore the expression of HDPD1 gene in EA.hy926 cell apoptosis induced by high glucose and hight cholesterol.Methods: cells were incubated with high or low concentrations of glucose(25 mmol/L) and cholesterol(3.2 mmol/L) for different times.We detected the cell activity by CCK8 Kit,observed the cell apoptosis by FACS(Flow Cytometer),and measured the expression of HSPD1 gene by RT-PCR and QPCR.Results: Cholesterol(0.8,1.6,3.2 mmol/L) together with high glucose strikingly decresed cell activity and in a dose-dependent manner,the same as the cholesterol(1.6,3.2 mmol/L) together with low glucose.The cell activity also decreased in a time dependent manner when treated with low or high glucose + 0.8 mmol/L.Most of cells treated with hight glucose and hight cholesterol(high glucose + 0.8 mmol/L cholesterol) underwent apoptosis compared with the low glucose and high cholesterol(high glucose + 0.8 mmol/L cholesterol).Meanwhile,the expression of HSPD1 gene were dramatically induced by high glucose and high cholesterol.