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| 富含高丰度蛋白的人参果实双向电泳图谱的建立 | |
| 引用本文： | 马朋涛,孙立伟,麻 锐,雷秀娟,陈雪楠,祁 超.富含高丰度蛋白的人参果实双向电泳图谱的建立[J].华中师范大学学报(自然科学版),2012,46(2):198-203. |
| 作者姓名： | 马朋涛 孙立伟 麻 锐 雷秀娟 陈雪楠 祁 超 |
| 作者单位： | 1. 华中师范大学生命科学学院,武汉,430079 2. 北华大学生命科学中心,吉林吉林,132013 3. 长春中医药大学药学院,长春,130117 4. 中国农业科学院特产研究所,吉林吉林,132109 |
| 基金项目： | 国家科技支撑计划项目，国家自然科学基金 |
| 摘 要： | 通过常规的TCA-丙酮沉淀法和丙酮沉淀法对人参果实蛋白的提取效果,确立了丙酮沉淀法更适合人参果实蛋白的提取.为进一步改善其2-DE分离效果,对丙酮沉淀法提取的总蛋白用Tris-饱和酚提纯,在其2-DE图谱效果有所改善的基础上,又在Tris-饱和酚中加入等体积的氯仿/异戊醇(24∶1)对丙酮沉淀法提取的总蛋白进行再次提纯,最终得到质量较高的2-DE图谱,其高、低丰度蛋白都得到了较好的分离. |
| 关 键 词： | 人参果实 蛋白提取 双向电泳 高丰度蛋白 低丰度蛋白 |
Establishment of 2-DE pattern of ginseng fruit, a tissue containing high levels of high abundant proteins |
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| MA Pengtao , SUN Liwei , MA Rui , LEI Xiujuan , CHEN Xuenan , QI Chao.Establishment of 2-DE pattern of ginseng fruit, a tissue containing high levels of high abundant proteins[J].Journal of Central China Normal University(Natural Sciences),2012,46(2):198-203. | |
| Authors: | MA Pengtao SUN Liwei MA Rui LEI Xiujuan CHEN Xuenan QI Chao |
| Institution: | 1(1.College of Life Science,Huazhong Normal University,Wuhan 430070; 2.Life Science Research Center,Beihua University,Jilin,Jilin 132013; 3.College of Pharmacy,Changchun University of Traditional Chinese Medicine,Changchun 130023; 4.Institute of Special Wild Economic Animal and Plant Science,Chinese Academy of Agricultural Sciences,Jilin,Jilin 132109) |
| Abstract: | In this paper,TCA-acetone precipitation method and acetone precipitation method were firstly compared and the latter was fixed to better suit the separation for the proteins of ginseng fruit.Then,the proteins extracted by the acetone precipitation method were optimized by the Tris-saturated phenol,which results in better improvement of the two-dimensional electrophoresis(2-DE) pattern.On this basis,equivoluminal chloroform/isoamyl alcohol(24∶1) was added to the Tris-saturated phenol to optimize the proteins extracted by the acetone precipitation method,and high-quality 2-DE was establish ultimately,which contains better separated spots of both higher and lower abundant proteins.It will lay the foundation for the proteomic analysis of ginseng fruit,and also provide a reference for 2-DE separation of proteins from similar tissues |
| Keywords: | ginseng fruit protein extraction 2-DE high abundant protein low abundant protein |
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