| 地中海伞藻(Acetabularia mediterranea)叶绿体DNA串联重复顺序的克隆和限制性内切酶图谱分析 |
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| 引用本文： | 柴建华.地中海伞藻(Acetabularia mediterranea)叶绿体DNA串联重复顺序的克隆和限制性内切酶图谱分析[J].复旦学报(自然科学版),1990(4). |
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| 作者姓名： | 柴建华 |
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| 作者单位： | 复旦大学遗传学研究所 |
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| 摘 要： | 以COS质粒(cosmid)pCOS 2EMBL 为载体,构建了地中海伞藻叶绿体基因组分子克隆库,并且筛选到含有串联重复顺序的克隆。经限制性内切酶酶解图谱分析,证明了串联重复顺序的存在,重复次数为4次以上。
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| 关 键 词： | 伞藻 叶绿体 COS质粒分子 串联重复顺序 限制性内切酶图谱 |
MOLECULAR CLONING AND RESTRICTION MAPPING OF TANDEMLY REPETITIVE SEQUENCE OF Acetabularia mediterranea CHLOROPLAST GENOME |
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| Chai Jianhua,.MOLECULAR CLONING AND RESTRICTION MAPPING OF TANDEMLY REPETITIVE SEQUENCE OF Acetabularia mediterranea CHLOROPLAST GENOME[J].Journal of Fudan University(Natural Science),1990(4). |
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| Authors: | Chai Jianhua |
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| Institution: | Institute of Genetics |
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| Abstract: | The chloroplast genomic library of Acetabularia mediterranea w as constructedby using the cosmid pCOS 2EMBL as vector. The cosmid clones containing tandemlyrepetitive sequence were selected. The presence of the tandemly repetitive sequencewas demonstrated by restriction mapping and its repcated number was 4 or more. |
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| Keywords: | Acetabularia chloroplast cosmid library tandemly repetitive sequence restriction mapping |
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