高温烃降解菌在含油污水生化处理中的应用

为强化油田污水的生化处理效果，从胜利油田采出液中筛选出3株高温烃降解菌，分别编号为JQ-1、JQ-2、JQ-3，初步鉴定JQ-1、JQ-2为芽孢杆菌属，JQ-3为不动杆菌属，其原油降解率分别为48%、55%、46%。将3株菌混合，其对原油的降解效果好于单株菌，其降解率可达到70%以上，混合菌降解原油的适宜条件分别是温度为45~55℃、pH值为5.5~6.5、矿化度12000~18000mg/L。在室内模拟现场条件进行污水生化处理试验，结果表明：含油量为40mg/L左右的油田污水经过8h处理后含油量在2mg/L以下，同时能有效的抑制硫酸盐还原菌的生长。该混合菌在一定程度上提高了含油污水生化处理的可行性，为油田污水治理提供了理论基础。

APPLICATION OF HIGH TEMPREATURE HYDROCARBON DEGRADATION BACTERIA ON BIOCHEMICAL TREATMENT TO PRODUCED OILY WATER

In order to improve the biochemical treating effect of oilfield produced water, three bacteria strains, JQ-1, JQ-2, JQ-3, are isolated from produced fluids in Shengli oilfield, Which oil degrading rate are up to 48%, 55% and 46% respectively. Strains JQ-1, JQ-2 are identified as Bacillus sp, JQ-3 as Acinetobacter sp. Comparing with single bacterium, mixed three bacteria strains have better degradation ability, whose degrading rate of crude oil is up to 70%, the best degradation conditions of mixed bacteria are: temperature 45~55℃, pH6.5~7.0, mineralized rate 12000~18000mg/L. The bench scale laboratory biochemical test simulating field conditions show that after 8 hours, the oil content of oilfield produced water is reduced from 40mg/L average to less than 2mg/L, at the same time, sulfate reducing bacteria (SRB) is restrained effectively. Continuous biochemical treatment with hydrocarbon degradation bacteria is improved.