Cellular uptake of amelogenin,and its localization to CD63, and Lamp1-positive vesicles |
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Authors: | J. L. Shapiro X. Wen C. T. Okamoto H. J. Wang S. P. Lyngstadaas M. Goldberg M. L. Snead M. L. Paine |
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Affiliation: | (1) Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry, 2250 Alcazar Street, CSA room 103, Los Angeles, CA 90033-1004, USA;(2) Department of Pharmaceutical Sciences, University of Southern California School of Pharmacy, Los Angeles, CA 90089-9121, USA;(3) Department of Biomaterials, Faculty of Dentistry, University of Oslo, P. O. Box 1109 Blindern, 0317, Oslo, Norway;(4) Faculté de Chirurgie Dentaire, EA 2496 Groupe ’Matrices extracellulaires et biominéralisations’, Université Paris 5, 1 rue Maurice Arnoux, 92120 Montrouge, France |
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Abstract: | Proteins of the developing enamel matrix include amelogenin, ameloblastin and enamelin. Of these three proteins amelogenin predominates. Protein-protein interactions are likely to occur at the ameloblast Tomes’ processes between membrane-bound proteins and secreted enamel matrix proteins. Such protein-protein interactions could be associated with cell signaling or endocytosis. CD63 and Lamp1 are ubiquitously expressed, are lysosomal integral membrane proteins, and localize to the plasma membrane. CD63 and Lamp1 interact with amelogenin in vitro. In this study our objective was to study the molecular events of intercellular trafficking of an exogenous source of amelogenin, and related this movement to the spatiotemporal expression of CD63 and Lamp1 using various cell lineages. Exogenously added amelogenin moves rapidly into the cell into established Lamp1-positive vesicles that subsequently localize to the perinuclear region. These data indicate a possible mechanism by which amelogenin, or degraded amelogenin peptides, are removed from the extracellular matrix during enamel formation and maturation. Received 27 September 2006; received after revision 24 November 2006; accepted 5 December 2006 |
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Keywords: | Amelogenin CD63 Emdogain? enamel endocytosis Lamp1 late endosome protein-protein interactions |
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