| 反式激活HIV-1 LTR的HHV-6基因片段B701的缺失研究 |
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| 引用本文： | 魏莘,张莉,刘淑红,陈启民,耿运琪.反式激活HIV-1 LTR的HHV-6基因片段B701的缺失研究[J].南开大学学报,1999,32(1):7-13. |
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| 作者姓名： | 魏莘 张莉 刘淑红 陈启民 耿运琪 |
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| 作者单位： | 南开大学生命科学学院 |
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| 基金项目： | 国家科委基础研究高技术司资助 |
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| 摘 要： | ＨＨＶ－６在体外可以激活ＨＩＶＬＴＲ引导的基因表达［１］，其基因组中一基因片段Ｂ７０１已被证明与这种激活作用有关［２］．Ｂ７０１编码１４３个氨基酸的蛋白质，可与ＨＨＶ－６基因组中其它基因片段协同作用提高对ＨＩＶＬＴＲ的激活效力．对Ｂ７０１进行缺失突变，得到突变体ＲＳＶ－Ｂ７０１－ｄ１（３′端缺失１８１个ｂｐ）、ＲＳＶ－Ｂ７０１－ｄ６（３′端缺失３５３个ｂｐ），将这两个缺失突变体分别与ＨＩＶ－Ｌｕｃ共转染受体细胞，通过ＬＵＣ活性分析发现，缺失突变体ｄ１、ｄ６不但仍具有激活能力，而且ｄ６的激活能力要远远大于ｄ１．二级结构预测初步揭示了Ｂ７０１对ＨＩＶ－ＬＴＲ的反式激活作用机制，为阐明ＨＨＶ－６基因产物与ＡＩＤＳ的病理关系提供了依据．
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| 关 键 词： | 缺失突变 反式激活 |
STUDY ON DELETION OF HHV-6 GENE SEGMENT B701 WHICH CAN TRANS-ACTIVATE HIV-1 LTR |
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| C.Wood,Wei Xin,Zhang Li,Liu Shuhong,Chen Qimin,Geng Yunqi,C.Wood.STUDY ON DELETION OF HHV-6 GENE SEGMENT B701 WHICH CAN TRANS-ACTIVATE HIV-1 LTR[J].Acta Scientiarum Naturalium University Nankaiensis,1999,32(1):7-13. |
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| Authors: | CWood Wei Xin Zhang Li Liu Shuhong Chen Qimin Geng Yunqi CWood |
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| Abstract: | Human Herpesvirus (HHV 6) can trans activate the human immunodeficiency virus (HIV) promoter and accelerate cytopathetic effects in HIV infected human T cells. A gene segment B701 showed high trans active ability in vitro. In this study, we demonstrated that 2 deletions of B701 can trans activate the HIV promoter in contransfected CV 1 cell line. All these deletions cannot trans activate HIV promoter constructs containing a deleted or mutant enhancer site. We also identified that B701 can work together with other gene segments that can trans activate HIV 1 LTR in vitro to increase the HIV LTR directed Luc gene expression dramatically. |
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| Keywords: | HHV-1 HHV-6 |
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