Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy |
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Authors: | XiaoJun Fan Bo Zheng YueJun Fu Yi Sun AiHua Liang |
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Affiliation: | (1) Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, China |
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Abstract: | An Buthus martensii Karsch Insect Toxin (BmK IT) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1 × 107 PlBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6 h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8 h) during a 192 h infection. Supported by the National Natural Science Foundation of China (Grant Nos. 30670282 and 30700534) and the Department of Science and Technology of Shanxi Province |
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Keywords: | scorpion neurotoxin BmK IT recombinant baculovirus expression bioassay |
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