| 新鲜哈密瓜汁中芽胞菌的分离鉴定及超高压对其致死效应研究 |
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| 引用本文： | 姜海荣,尹琳琳,宋丽军,于小燕,陈计峦.新鲜哈密瓜汁中芽胞菌的分离鉴定及超高压对其致死效应研究[J].石河子大学学报,2010,28(1):91-95. |
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| 作者姓名： | 姜海荣 尹琳琳 宋丽军 于小燕 陈计峦 |
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| 作者单位： | 石河子大学食品学院; |
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| 基金项目： | 教育部重点课题(207137);;兵团博士基金项目(2007jc11) |
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| 摘 要： | 为探讨哈密瓜汁超高压杀菌的新方法,以新鲜哈密瓜为试验材料,通过形态特征的传统鉴定方法分离得到2株芽胞菌,采用细菌16SrRNA基因的通用引物进行PCR并扩增出这2株菌的16SrRNA基因全序列。测定16SrRNA基因序列,将获得的序列用blast软件与Genbank中相关菌株的16SrDNA序列进行同源性分析,结果显示两菌株均为枯草芽孢杆菌。采用100~500MPa超高压协同35~55℃处理5~20 min进行枯草芽孢杆菌的杀菌实验,结合哈密瓜汁的理化指标和最大程度保持哈密瓜汁原有特殊风味,得出45℃、300MPa、保压15min为杀菌的最优参数,能使哈密瓜汁中的枯草芽孢杆菌降低5.23个对数。
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| 关 键 词： | 芽胞菌 分离鉴定 超高压 杀菌 哈密瓜汁 |
A Research into the Lethal Effect of Bacillus sp.Isolated and Identified from Fresh Hami Melon Juice with high Hydrostatic Pressure Treatment |
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| JIANG Hairong,YIN Linlin,SONG Lijun,YU Xiaoyan,CHEN Jiluan.A Research into the Lethal Effect of Bacillus sp.Isolated and Identified from Fresh Hami Melon Juice with high Hydrostatic Pressure Treatment[J].Journal of Shihezi University(Natural Science),2010,28(1):91-95. |
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| Authors: | JIANG Hairong YIN Linlin SONG Lijun YU Xiaoyan CHEN Jiluan |
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| Institution: | Food College;Shihezi University;Shihezi 832003;China |
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| Abstract: | Fresh Hami-melon juice was used as test material in this study to isolate the Bacillus subtilis.After the traditional identification of morphological features investigation,the 16SrDNA sequences were performed by employing the universal prime bacterial for polymerase chain reaction(PCR) amplification.The alignment search was performed with BLAST and GenBank databases.Compared the software of Clastal1.81,the phylogenetic tree was built using Mega 4.1 software.From the phylogenetic tree,it can be seen that Ba... |
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| Keywords: | Bacillus sp isolate and identify high hydrostatic pressure treatment fresh Hami-melon juice |
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