不醉丹的有效性研究

目的观察不醉丹对醉酒小鼠的解酒作用,初步探讨其解酒机制。方法①雄性BALB/c小鼠30只,随机分为生理盐水组、海王金樽组和不醉丹组,每只小鼠分三次二锅头酒灌胃,每次0.06mL,共0.18mL。每次给酒后各组分别随即用生理盐水、海王金樽和不醉丹0.06mL/只灌胃,观察记录第一次给酒后至醉酒状态出现所需时间以及发生醉酒的动物数量;②重复上述试验,各组小鼠于初次给酒后50min采血分离血浆,检测酒精浓度。结果①不醉丹组和海王金樽组发生醉酒的动物数量均为2/10只,明显少于生理盐水组的9/10只。醉酒状态出现所需平均时间,不醉丹组和海王金樽组与生理盐水组比较差异不显著;②不醉丹组小鼠血浆酒精浓度明显低于生理盐水组(P<0.05)。结论不醉丹能有效改善小鼠的醉酒状态,其作用机理可能与降低血浆酒精浓度相关。

Alcohol-dispelling Effect of BUZUIDAN and Its Underlying Mechanism

Objective To observe alcohol-dispelling effect of BUZUIDAN on drunken mice,and explore the mechanism of action.Methods: ①Male BALB/c mice were divided into three groups randomly: normal saline group,KingDrink group and BUZUIDAN group.Each mouse was administered with Beijing spirit er guo tou by gastric perfusion for three times,0.06mL once at 10 minutes intervals,and the total volume was 0.18mL.Following each alcohol administration,0.06ml of normal saline,KingDrink and BUZUIDAN were respectively fed to each mouse of the corresponding group immediately by gastric perfusion.The time period were recorded for each mouse getting drunk after the first alcohol administration,as well as the number of drunken mice.②Repeating the experiments mentioned above,effects on plasma alcohol concentration in mice.Blood were collected and plasma was dissociated from each mouse in 50 minutes following the first alcohol administration,and then alcohol concentration was determined.Results: ① The number of the drunken animals was 2/10 both in BUZUIDAN group and in KingDrink group,which was obviously less than that in the normal saline group with 9/10 drunken animals.There is no significant difference in the mean time of animal getting drunk when the BUZUIDAN group and KingDrink group were compared to the normal saline group;②Plasma alcohol concentration of the mice in the BUZUIDAN group was lower than that in the normal saline group(P< 0.05)significantly.Conclusion: BUZUIDAN can effectively relieve drunkness in mouse,and the mechanism of effects was possibly associated with lowering plasma alcohol concentration.