Localization of Sry gene on Y chromosome of Muntjac munticus vaginalis |
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Authors: | ZHANG Yue SHAN Xiangnian Liu Ningsheng LU Xiaoxuan NIE Wenhui YANG Fengtang Wang Jinhuan Chen Yuze |
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Affiliation: | 1. Medical Genetics Research Center, Medical College, Southeast University, 2. Kunming Institute of Zoology, Chinese Academy of Sciences, |
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Abstract: | The chromosomes 1, Y1, Y2 of Muntjac munticus vaginalis were isolated by fluorescence activated chromosome sorting and amplified by degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR). A primer pair within human Sry HMG box was designed and the Sry gene of the male M. m vaginalis was amplified. The product was cloned and sequenced. The result proved that Sry is located on chromosome Y2, which is the sex-determining chromosome in the male M. m vaginalis. |
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