Genetic typing of mitochondrial DNA in sheep (Ovis aries ) |
| |
Institution: | 1. College of Animal Science and Technology, China Agricultural University, Beijing 100094, China 2. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China |
| |
Abstract: | Using PCR-RFLP and DNA sequencing, this study confirmed that HinfI polymorphism in the ovine mitochondrial COI gene resulted from the T-C substitution at the nucleotide 234 but this mutation did not encode another amino acid, which was actually a synonymous mutation. This single nucleotide polymorphism can be used as gene typing marker for mitochondrial genome in the research of the interactions between mitochondria and nucleus, extranuclear gene effects, and as molecular discriminating marker for embryo or individual in the research area of gene transfer and animal cloning. |
| |
Keywords: | sheep mtDNA typing single nucleotide polymorphism |
本文献已被 万方数据 等数据库收录! |
| 点击此处可从《自然科学进展(英文版)》浏览原始摘要信息 |
| 点击此处可从《自然科学进展(英文版)》下载免费的PDF全文 |
|