A point
mutation of PRL gene in pituitary prolactin-secreting adenoma induced by 17-b-estradiol in
SD rats |
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作者单位: | Department of Physiology,Institute of Basic Medical Science,Chinese Academy of Medical Sciences,Beijing 100005,China
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摘 要: |
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收稿时间: | 24 May 1999 |
A point mutation of PRL gene in pituitary prolactin-secreting adenoma induced by 17-β-estradiol in SD rats |
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Authors: | Xuemei Wu Rongkun Xu |
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Institution: | (1) Department of Physiology, Institute of Basic Medical Science, Chinese Academy of Medical Sciences, 100005 Beijing, China |
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Abstract: | Animal model bearing pituitary prolactin-secreting adenomas (prolactinoma) induced by 17-β-estradiol (E2) in both eutopic pituitary and ectopic pituitary grafted under the renal capsule was generated. Northern blotting assay indicated
that PRL mRNA level in eutopic prolactinomas was higher than that in normal pituitaries and ectopic prolactinomas (P < 0.05-0.01). By polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) analysis and DNA sequencing,
a point mutation from C to A occurring at -36 nt in proximal promoter of rat PRL (rPRL) gene was found only in eutopic prolactinomas.
No base change was detected in ectopic prolactinomas. Fusion gene transfection assayin vitro exhibited increased activity of the mutant promoter derived from eutopic prolactinoma (P < 0.01). These data suggested that the base change in the proximal promoter of rPRL gene may be associated with hyperexpression
of rPRL gene in eutopic prolactinomas. The pathogenesis of eutopic and ectopic prolactinomas induced by E2 in SD rats may be separate. |
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Keywords: | PCR-SSCP prolactin-secreting adenoma rPRL gene DNA sequencing point mutation |
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