| 血清抗Sm抗体ELISA检测方法的建立 |
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| 引用本文： | 曾常茜,张晶波,曾常明,朴今子,赵云冬.血清抗Sm抗体ELISA检测方法的建立[J].北华大学学报(自然科学版),1999(1). |
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| 作者姓名： | 曾常茜 张晶波 曾常明 朴今子 赵云冬 |
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| 作者单位： | 吉林医学院免疫学研究室!吉林132001(曾常茜)，吉林医学院微生物教研室(张晶波)，吉林省石岘造纸厂职工医院(曾常明，朴今子)，吉林卫校(赵云冬) |
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| 摘 要： | 目的：建立血清抗Sm抗体的ELISA检测方法。方法：应用纯化的Sm抗原，采用棋盘滴定等方法确立ELISA方法检测血清抗Sm抗体的最适实验条件。结果：建立了血清抗Sm抗体的ELISA检测方法。SLE患者血清抗Sm抗体的阳性率为29．2％，正常人和其它自身免疫病患者未查到此抗体。结论：本文建立的血清抗Sm抗体检测方法敏感性达到国外进口试剂盒的水平，且操作简便，重复性好，因此临床很有实用价值。
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| 关 键 词： | 抗Sm抗体 ELISA SLE |
Establishment of ELISA to detect anti-Sm antibody in Srum |
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| Zeng Changqian, et al.Establishment of ELISA to detect anti-Sm antibody in Srum[J].Journal of Beihua University(Natural Science),1999(1). |
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| Authors: | Zeng Changqian |
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| Abstract: | objective: To establish ELISA for anti - Sm antibody in serum. Methods: Using purified SmAg and Chess Board Titraing method, we set up the optimal experimental conditions of FLISA for anti - Sm antibody. Result: The ELISA for anti- Sm antibody in serum from SLE was established. The positive rate of anti - Sm antibody in serum from SLF was 29. 2%. There was no anti - Sm antibody in normal and other autoimmune disease controls. Conclusion: The sensitivity of the ELISA for anti -Sm antibody approched to abroad ELISA Kit. In addition, it is easy and reproducible. so, it is practical clinically. |
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| Keywords: | anti-smantibody ELISA SLE |
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