Untersuchungen über die Reinigung des Protektins Anti-Ahel (Anti-AHP) ausHelix pomatia |
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Authors: | O. Kühnemund W. Köhler |
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Affiliation: | (1) Institut für Mikrobiologie und experimentelle Therapie, Deutsche Akademie der Wissenschaften zu Berlin, 69 Jena, DDR |
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Abstract: | Summary A method is described which allows separation of the protectin Anti Ahel (Anti AHP from crude extracts of the protein gland ofHelix pomatia. A solution of the crude extract in 0.05M sodium phosphate buffer, pH 7.0, is applicated on a Sephadex G 200 column. The protectin is retained on the gel matrix, while the other components of the mixture are eluted. The protectin is eluted with a 0.5M solution of glucose in buffer, and is isolated by dialyzation against aqua dest. and lyophilization.
Frau R.John und Frau B.Hoffmann sind wir für die gewissenhafte Durchführung der experimentellen Arbeiten zu Dank verpflichtet. |
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