| 山苍子枝和叶中总黄酮含量的测定 |
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| 引用本文： | 陈湛娟,刘秀萍,毕和平,肖宝东.山苍子枝和叶中总黄酮含量的测定[J].海南师范大学学报(自然科学版),2012,25(4):409-411. |
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| 作者姓名： | 陈湛娟 刘秀萍 毕和平 肖宝东 |
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| 作者单位： | 1. 海南师范大学化学与化工学院,“省部共建一热带药用植物化学”教育部重点实验室,海南海口571158
2. 晋中学院化学化工学院,山西晋中,030600 |
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| 摘 要： | 以芦丁标准品为对照,采用NaNO2-A（l NO3）3-NaOH显色系统,紫外分光光度法测定山苍子枝和叶中黄酮类物质的含量.试验结果表明,山苍子枝和叶中总黄酮含量分别为1.09%,2.14%.加标回收率分别为100.70%和104.30%,RSD分别为3.14%和1.94%.本法简单易操作,结果准确,可靠.
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| 关 键 词： | 山苍子 总黄酮 含光光度法 |
Determination of Total Flavonoid Content in the Stems and Leaves of Litsea cubeba. |
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| Institution: | CHEN Zhanjuan1, LIU Xiuping2, BI Hepingl1, XlAO Baodong1 ( 1. College of Chemisty and Chemical Engineering,Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, China; 2. College of Chemistry and Chemical Engineering, Jinzhong University, Jinzhong 030600, China) |
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| Abstract: | To establish the determination methods of the total flavonoids contents from Litsea cubeba, Took rutin as standard, contents of total flavonoids in Litsea cubeba stems and leaves were determined by spectrophotometer with Na- NO2-AI （NO3）3-NaOH as colored reagent. The average value of contents of total flavonoids in Litsea cubeba stems and leaves were 1.09% and 2.14%. The average recovery of the method were 100.7% and 104.3%, the RSD of reproducibility test were 1.45% and 0.62% （n=5） respectively. The method is simple and easy to operate, the result is accurate and reliable. |
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| Keywords: | Litsea cubeba Flavonoids UV |
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