A mammalian protein with specific demethylase activity for mCpG DNA |
| |
Authors: | Bhattacharya S K Ramchandani S Cervoni N Szyf M |
| |
Institution: | Department of Pharmacology and Therapeutics, McGill University, Montreal, QC, Canada. |
| |
Abstract: | DNA-methylation patterns are important for regulating genome functions, and are determined by the enzymatic processes of methylation and demethylation. The demethylating enzyme has now been identified: a mammalian complementary DNA encodes a methyl-CpG-binding domain, bears a demethylase activity that transforms methylated cytosine bases to cytosine, and demethylates a plasmid when the cDNA is translated or transiently transfected into human embryonal kidney cells in vitro. The discovery of this DNA demethylase should provide a basis for the molecular and developmental analysis of the role of DNA methylation and demethylation. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|