| 固相萃取-高效液相色谱法测定人体血清中游离皮质醇 |
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| 引用本文： | 石红梅,徐向东,时婧,赵心,朱鹤,康维钧.固相萃取-高效液相色谱法测定人体血清中游离皮质醇[J].河北大学学报(自然科学版),2009,29(4). |
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| 作者姓名： | 石红梅 徐向东 时婧 赵心 朱鹤 康维钧 |
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| 作者单位： | 1. 河北医科大学,公共卫生学院,河北,石家庄,050017
2. 河北医科大学第三医院,麻醉科,河北,石家庄,050017 |
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| 基金项目： | 河北省科学技术研究与发展指导项目 |
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| 摘 要： | 使用C18固相萃取小柱纯化、富集人体血清中的游离皮质醇,C18反相高效液相色谱法分离,V(甲醇)∶V(0.02 mol/L乙酸铵)∶V(乙酸)∶V(三乙胺)=60∶40∶0.5∶0.5为流动相,波长242 nm下紫外检测器检测,建立了一种简单、灵敏的人体血清中游离皮质醇的检测方法.该方法测定皮质醇的线性范围为(0.1~3.0)×10-6g/mL,标准曲线线性相关系数为0.997 9,方法检出限为2.5×10-8g/mL,样品加标回收率为86.0%~91.7%.测定了8个人体麻醉前后的血样样本皮质醇含量.该方法准确,稳定,有良好的重现性和较高的灵敏度.
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| 关 键 词： | 固相萃取 高效液相色谱 皮质醇 血清 |
SPE-HPLC Determination of Free Cortisol Levels in Human Serum |
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| SHI Hong-mei,XU Xiang-dong,SHI Jing,ZHAO Xin,ZHU He,KANG Wei-jun.SPE-HPLC Determination of Free Cortisol Levels in Human Serum[J].Journal of Hebei University (Natural Science Edition),2009,29(4). |
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| Authors: | SHI Hong-mei XU Xiang-dong SHI Jing ZHAO Xin ZHU He KANG Wei-jun |
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| Institution: | 1.School of Public Health;Hebei Medical University;Shijiazhuang 050017;China;2.Anesthesiology Department;The Third Hospital of Hebei Medical University;China |
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| Abstract: | A simple and sensitive analytical method is described for the determination of free cortisol in human serum using SPE-C18 clean-up and enrichment,coupled with high performance liquid chromatography with UV-detection.The mobile phase was methanol,aceticamine(0.02 mol/L),acetic acid and triethylamine(TEA),and volume ratio was 60∶40∶0.5∶0.5.The calibration curve of cortisol was linear(r=0.997 9) in the range of(0.1~3.0)×10-6 g/mL.The detection limit was 2.5×10-8 g/mL,and the recovery of cortisol was 86.0%~91.7%.The method was used for the analysis of cortisol in eight blood samples before and after narcotism.The proposed method is precise and suitable for the detection of free crotisol in serum. |
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| Keywords: | solid-phase extraction HPLC cortisol serum |
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