| 甘草水通道蛋白PIP1基因正义和反义表达载体的构建 |
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| 引用本文： | 王芳,董乐,黄周英. 甘草水通道蛋白PIP1基因正义和反义表达载体的构建[J]. 内蒙古民族大学学报(自然科学版), 2008, 23(6): 642-646 |
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| 作者姓名： | 王芳 董乐 黄周英 |
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| 作者单位： | 泉州师范学院,化学与生命科学学院,福建,泉州,362000 |
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| 基金项目： | 福建省科技厅青年科技人才创新项目，福建省自然科学基金高校专项项目，福建省教育厅科技项目，泉州市科技局技术研究与开发项目，泉州师范学院大学生基金 |
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| 摘 要： | 根据已报道的GuPIP1基因序列设计特异引物，克隆甘草水通道蛋白GuPIP1基因cDNA序列，将该片段正向、反向分别插入植物表达载体pMBW330的CaMV35S启动子和OCS终止子之间，构建了正义、反义表达载体．通过双酶切、PER和DNA测序鉴定后，分别导入农杆菌EHA105中．
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| 关 键 词： | 甘草 水通道蛋白 GuPIP1 表达载体 |
Construction of Expression Vector of Sense and Antisense GuPIP1 Gene in Chinese Licorice(Glycyrrhiza uralensis F.) |
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| WANG Fang,DONG Le,HUANG Zhou-ying. Construction of Expression Vector of Sense and Antisense GuPIP1 Gene in Chinese Licorice(Glycyrrhiza uralensis F.)[J]. Journal of Inner Mongolia University for the Nationalities(Natural Sciences), 2008, 23(6): 642-646 |
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| Authors: | WANG Fang DONG Le HUANG Zhou-ying |
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| Affiliation: | WANG Fang, DONG Le, HUANG Zhou - ying (Department of Chemical ＆ Biology Science ,Quanzhou Normal University,Quanzhou 362000,China) |
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| Abstract: | Specific primer was designed according and the eDNA sequence of GuPIP1 was obtained. to the reported eDNA sequence of the GuPIPI, GuPIP1 sense and antisense sequence was then inserted between the CaMV 35S promoter and COS terminator into the expression vector pMBW330 respectively. The expression vector was checked then with two enzymes cutting, PCR analysis and sequencing. Then recombination vector was transformed into Agrobacterium tumefaciens EHA 105 respectively. |
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| Keywords: | GuPIP1 |
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