| ~(99m)Tc间接标记单克隆抗体的研究 |
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| 引用本文： | 贾芳,孟昭兴,蒋燕,王华,刘伯里.~(99m)Tc间接标记单克隆抗体的研究[J].北京师范大学学报(自然科学版),2000(2). |
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| 作者姓名： | 贾芳 孟昭兴 蒋燕 王华 刘伯里 |
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| 作者单位： | 北京师范大学化学系!100875，北京，27岁，女，博士生，北京师范大学化学系!100875，北京，北京师范大学化学系!100875，北京，北京师范大学化学系!100875，北京，北京师范大学化学系!100875，北京 |
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| 摘 要： | 以NHS MAG3为双功能连接剂 ,采用先标记后偶联的方法 ,研究了用99mTc标记单克隆抗体的各种实验条件 ,得到了最佳标记方法和偶联方法 .标记抗体99mTc NHS MAG3 IgG体外稳定性良好 .进行了99mTc NHS MAG3 CL3在小鼠体内的生物分布测定 .结果表明 ,该配合物在小鼠体内稳定性良好 ,在胃、肠、心中摄取最低 ,在肾 ,肝中摄取最高 .
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| 关 键 词： | ~(99m)Tc标记单克隆抗体 NHS-MAG_3 双功能连接剂 偶联 生物分布 |
A STUDY ON INDIRECT METHOD FOR ~(99m)Tc LABELING MONOCLONAL ANTIBODY |
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| Jia Fang,Meng Zhaoxing,Jiang Yan,Wang Hua,Liu Boli.A STUDY ON INDIRECT METHOD FOR ~(99m)Tc LABELING MONOCLONAL ANTIBODY[J].Journal of Beijing Normal University(Natural Science),2000(2). |
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| Authors: | Jia Fang Meng Zhaoxing Jiang Yan Wang Hua Liu Boli |
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| Abstract: | The experimental conditions of 99m Tc labeling with antibodies via NHS MAG 3 as a bifunctional chelating agent are studied by pre radiolabeling of chelate approach. The conjugation conditions are optimized for the best conjugation performance.The stability of 99m Tc NHS MAG 3 IgG in vitro and 99m Tc NHS MAG 3 CL3 in vivo is high. The uptake of 99m Tc NHS MAG 3 CL3 is low in stomach, intestine and heart, and high in liver and kidney, respectively. |
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| Keywords: | m Tc monoclonal antibodies NHS MAG 3 bifunctional chelating agent conjugation biodistribution |
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