利用离体培养技术打破桂花种子休眠的试验

<正>桂花种子成熟后胚有后熟作用,胚乳中可能含有抑制胚萌发的物质,使用赤霉素和3个月的层积处理也不能促使其萌发。将桂花种子进行3个月层积处理后,进行离体培养,培养基中加入0.5 mol/L BA,1.0 mol/L BA+0.5NAA可以打破种子的休眠,促进萌发。

Preliminary Study on Breaking the Seed Dormancy of Osmanthus fragrans by in Vitro Culture

Osmanthus fragrans embryo has after-ripening when the seed is mature. There is perhaperps restraining substances in endosperm. The seed does not germinate even if it is dealed with GA or stored for three months in sand. By in vitro culture and adding 0.5 mol/L BA,1.0 mol/L BA+0.5 mol/L NAA to culture media, the seed dormancy can be broken after stored for three months in sand.