| 凡纳滨对虾在不同实验条件下感染白斑综合症的研究 |
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| 引用本文： | 潘忠诚,王芳宇,何建国,赵志英,翁少萍,付小哲,陈国良. 凡纳滨对虾在不同实验条件下感染白斑综合症的研究[J]. 衡阳师专学报, 2008, 0(6) |
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| 作者姓名： | 潘忠诚 王芳宇 何建国 赵志英 翁少萍 付小哲 陈国良 |
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| 作者单位： | 哈尔滨工程大学化工学院环境工程系 中山大学生命科学院 衡阳师范学院生命科学系 广东湛江海茂公司 |
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| 基金项目： | 国家863资助项目(2003AA603011);; 国家自然科学基金(30300266);; 哈尔滨工程大学校基金(02100260728) |
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| 摘 要： | 本研究目的是探讨在不同实验条件下的凡纳滨对虾人工感染白斑病毒(White Spot Syndrome Virus,WSSV)的死亡率变化。研究结果如下,当凡纳滨对虾感染10-3、10-4、10-5WSSV(每尾注射剂量0.1mL),15天后的死亡率分别为(100±0)%,(78±2.3)%,(21±1.4)%。当养殖在高(100尾/m3)、中(50尾/m3)和低密度组(25尾/m3)的凡纳滨对虾感染10-4WSSV,15天后的死亡率分别为(91±2.7)%、(78±1.3)%、(63±1.9)%。在相同养殖密度下的凡纳滨对虾感染10-4WSSV,雌虾组和雄虾组15天后的死亡率分别为(67.3±1.83)%,(51±2.1)%(p<0.05),白天的死亡率比晚上的低,差异显著(p<0.05)。另外,凡纳滨对虾注射感染的死亡率与投喂感染的死亡率比较,差异不显著(p>0.05)。
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| 关 键 词： | 凡纳滨对虾 WSSV 巢式PCR 死亡率 人工感染 |
Study on Litopenaeus Vannamei Infected with White Spot Syndrome Virus under Different Experimental Conditions |
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| PAN Zhong-cheng,;,; WANG Fang-yu,; HE Jian-guo,; ZHAO Zhi-ying,; WENG Shao-ping,; FU Xiao-zhe,; CHEN Guo-liang. Study on Litopenaeus Vannamei Infected with White Spot Syndrome Virus under Different Experimental Conditions[J]. Journal of Hengyang Normal University, 2008, 0(6) |
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| Authors: | PAN Zhong-cheng, , WANG Fang-yu, HE Jian-guo, ZHAO Zhi-ying, WENG Shao-ping, FU Xiao-zhe, CHEN Guo-liang |
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| Affiliation: | 1.Department of Environmental and Engineering; School of Material Science and Chemical Engineering; Harbin Engineering University; Harbin 150001; China; 2.State Key Laboratory for Biocontrol; School of Life Sciences; Zhongshan University; Guangzhou 510275; 3.Department of Life Science; Hengyang Normal University; Hengyang Hunan 421008; 4.Haimao Corporation of Zhanjiang; Zhanjiang Guangdong 524039; China); |
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| Abstract: | This paper highlights changes in mortalities of Litopenaeus vannamei challenged with WSSV under the different conditions.The results were shown as the following.When shrimps were challenged with 10-3,10-4 or 10-5 WSSV stock solution(0.1mL.shrimp-1),the cumulative mortalities on day 15 were(100±0) %,(78±2.3) %,and(21±1.4) %,respectively.When L.vannamei were challenged with 10-4 WSSV stock solution(0.1mL.shrimp-1) and then reared at the high-density(100 shrimps.m-3),the middle-density(50 shrimps.m-3),and the ... |
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| Keywords: | Litopenaeus vannamei WSSV Nested PCR morality artificial infection |
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