A rapid and simple method for the isolation of pure eosinophilic leukocytes from horse blood |
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Authors: | A Jörg P Portmann G Fellay J L Dreyer J Meyer |
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Institution: | (1) Physiologisch-chemisches Institut, Universität Freiburg, CH-1700 Freiburg, (Switzerland) |
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Abstract: | Summary An improved and short method is described for the isolation of intact eosinophilic leukocytes from horse blood with high yield (1–1.5 g/20 1). Viability and purity of the preparations were verified by light and electron microscopy and by the trypan blue exclusion test. Isolated eosinophils were 98–100% pure, intact and viable, and they could be shown to phagocytise immune-complexes.The authors are indebted to the Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung for financial support. |
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