响应面法优化醋酸菌在梨渣中的发酵条件研究

测定不同温度、转速、酒精浓度、接种量条件下醋酸菌在梨渣为主的半固体培养基中的产酸量,观察菌种的生长情况.采用响应面法优化醋酸菌生长条件.结果表明:醋酸菌较佳发酵条件为转速136 r.min-1,温度32℃,梨渣量28%,乙醇体积分数3.6%,接种量4.0%.

Optimization of Fermentation Conditions of Acetic Acid Bacteria in Pear Slag by Response Surface Analysis

Direct drinking vinegar,especially Fruit vinegar production is one of the most important way for deep processing of agricultural byproducts.This article is based on acetic acid bacteria was cultured under the conditions with different temperature,rotation rate,alcohol concentration,and inoculation.The total acid content was determined,and the growth of bacteria on the semisolid culture medium with pear slag was observed.Finally,response surface analysis was used to optimize the growth condition of acetic acid bacteria,and the optimum conditions were found as follows: rotation rate was 136 r·min-1,temperature was 32 ℃,the weight of the pear slag was 28%,alcohol content was 3.6%,inoculation was 4.0%.The result could provide a theory reference and practice basis for industrial production of the pear vinegar.