| Inhibitory Effect of Lithium Chloride on Mouse Thymocyte Apoptosis |
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| 作者姓名： | 田艳梅 李丕鹏 姜晓芳 张贵友 戴尧仁 |
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| 作者单位： | [1]DeDartmentofBiologicalSciencesandBiotechnology,TsinghuaUniversity,Beijing100084,China [2]DepartmentofBiology,YantaiNormalUniversity,Yantai264025,China |
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| 摘 要： | This study investigates the effect of lithium chloride (LiCI) on mouse thymocyte apoptosis. A primary culture of mouse thymocytes was preincubated with LiCI (from 5 to 500μmol/L) before exposure to dexamethasone (DEX), the apoptosis inducer. With 100μmol/L of LiCI, apoptotic cell death induced by DEX was almost completely prevented as determined by flow cytometric analysis, terminal deoxynudeotidyltransferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay and DNA laddering assay. The results show that the DEX-induced increment of caspase-3 activity in thymocytes is completelye liminated by LiCI preincubation. The results suggest that LiCI may protect Balb/c mouse thymocytes from apoptosis induced by glucocorticoid in a dose-dependent matter.
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| 关 键 词： | 鼠胸腺细胞 细胞凋亡 氯化锂 抑制作用 地塞米松 |
Inhibitory Effect of Lithium Chloride on Mouse Thymocyte Apoptosis |
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| Abstract: | This study investigates the effect of lithium chloride (LiCl) on mouse thymocyte apoptosis.A primary culture of mouse thymocytes was preincubated with LiCl (from 5 to 500 μmol/L) before exposure to dexamethasone (DEX), the apoptosis inducer.With 100 μmol/L of LiCl, apoptotic cell death induced by DEX was almost completely prevented as determined by flow cytometric analysis, terminal deoxynucleotidyltransferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay and DNA laddering assay.The results show that the DEX-induced increment of caspase-3 activity in thymocytes is completely eliminated by LiCl preincubation.The results suggest that LiCl may protect Balb/c mouse thymocytes from apoptosis induced by glucocorticoid in a dose-dependent matter. |
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| Keywords: | lithium chloride thymocyte apoptosis |
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