| Up—Regulation of CCR5 and CXCR4 Expression on Human Monocytes by Interferon Gamma |
| |
| 引用本文： | 陆韵,刘祖强,陈应华.Up—Regulation of CCR5 and CXCR4 Expression on Human Monocytes by Interferon Gamma[J].清华大学学报,2003,8(4):440-444. |
| |
| 作者姓名： | 陆韵 刘祖强 陈应华 |
| |
| 作者单位： | LaboratoryofImmunology,ResearchCenterforMedicalScience,DepartmentofBiologicalSciences.andBiotechnology,TsinghuaUniversity,Beijing100084,China |
| |
| 基金项目： | Supported by the National Key Basic Research Specific Funds (No. G19990 75 6 0 7) and the National Science
Foundation for Outstanding Young Scientist of China
(No. 30 0 2 5 0 38) |
| |
| 摘 要： | Chemokine receptors, mainly CCR5 and CXCR4, have been proved to be the important coreceptors in HIV-1 entry. HIV-1 disease progression is, in general, characterized by an initial predominance of CCR5 using macrophage tropic, non-syncytium-inducing (NSI) isolates, switching later to CXCR4 using T-cell tropic,syncytium-inducing (SI) isolates. How this shift occurs and how the shift can be controlled are still unclear.Since patients with rapid decline of T cell counts have constantly high levels of IFN-7 in the sera and lymphoidnodes, we investigated the influence of this cytokine on the expression of the HIV-1 coreceptors CCR5 and CXCR4 on the cell surfaces of human monocytic cell line U937 and promonocyte NB4. IFN-γ could intensively enhance the expression of both, while a low level of CCR5 expression was detected in two cell lines before stimulation. The results of semiquantitative RT-PCR also confirm the up-regulation. As the newly generated X4-strains have been demonstrated to be insensitive to chemokine in some reports, IFN-7 may play an important role in selecting CXCR4-used strains.
|
| 关 键 词： | 爱滋病 HIV－1 CCR5 CXCR4 人单核细胞 基因表达 干扰素 高端规定 |
Up-Regulation of CCR5 and CXCR4 Expression on Human Monocytes by Interferon Gamma |
| |
| LU Yun,LIU Zuqiang,CHEN Yinghua Laboratory of Immunology,Research Center for Medical Science.Up-Regulation of CCR5 and CXCR4 Expression on Human Monocytes by Interferon Gamma[J].Tsinghua Science and Technology,2003,8(4):440-444. |
| |
| Authors: | LU Yun LIU Zuqiang CHEN Yinghua Laboratory of Immunology Research Center for Medical Science |
| |
| Institution: | LU Yun,LIU Zuqiang,CHEN Yinghua ** Laboratory of Immunology,Research Center for Medical Science,Department of Biological Sciences and Biotechnology,Tsinghua University,Beijing 100084,China |
| |
| Abstract: | Chemokine receptors, mainly CCR5 and CXCR4, have been proved to be the important coreceptors in HIV-1 entry.HIV-1 disease progression is, in general, characterized by an initial predominance of CCR5 using macrophage tropic, non-syncytium-inducing (NSI) isolates, switching later to CXCR4 using T-cell tropic, syncytium-inducing (SI) isolates.How this shift occurs and how the shift can be controlled are still unclear.Since patients with rapid decline of T cell counts have constantly high levels of IFN-γ in the sera and lymphoid nodes, we investigated the influence of this cytokine on the expression of the HIV-1 coreceptors CCR5 and CXCR4 on the cell surfaces of human monocytic cell line U937 and promonocyte NB4.IFN-γ could intensively enhance the expression of both, while a low level of CCR5 expression was detected in two cell lines before stimulation.The results of semiquantitative RT-PCR also confirm the up-regulation.As the newly generated X4-strains have been demonstrated to be insensitive to chemokine in some reports, IFN-γ may play an important role in selecting CXCR4-used strains. |
| |
| Keywords: | HIV-1 chemokine receptor CXCR4 CCR5 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
