| 苯酚-硫酸法测定柳芽中多糖含量的研究 |
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| 引用本文： | 赵莹,刘飞,于之伦. 苯酚-硫酸法测定柳芽中多糖含量的研究[J]. 中国西部科技, 2014, 0(12): 64-65 |
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| 作者姓名： | 赵莹 刘飞 于之伦 |
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| 作者单位： | 泰山医学院药学院;泰安市食品药品检验检测中心; |
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| 基金项目： | 山东省医药卫生重点实验室培养基金项目 |
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| 摘 要： | 采用苯酚-硫酸法对柳芽中多糖含量按紫外分光光度法在482nm波长处测定吸光度,实验结果表明葡萄糖对照品在5.4~16.2μg/ml之间呈线性关系,该法简便易行,准确性高,重现性好。本文探讨了测定过程的影响因素,并通过此法测得柳芽中多糖含量为7.46%。
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| 关 键 词： | 柳芽 多糖 苯酚-硫酸法 含量 |
Application of Phenol-Sulfuric Acid Method in Determination of Polysaccharides in Willow Sprouts |
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| Affiliation: | ZHAO Ying, LIU Fei, YU Zhi-lun (1.School of Pharmaceutical Sciences, Taishan Medical University , Taian Shandong 271016,China;2.Taian Institute for Drug Control, Taian Shandong 271000,China) |
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| Abstract: | The content ofpolysaccharides in willow sprouts was determined by the method of phenol-sulfuric acid. The results showed a good linearity relationship between the absorbance and the content of standard glucose in the range of 5.4~ 16.2μg/ml. The method is simple, convenient and accurate, and has good reproducibility. The affecting factors in the assaying process were discussed in this paper respectively. By this method, the content of polysaccharides in willow sprouts was found to be 7.46% o |
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| Keywords: | Willow Sprouts Polysaccharides Phenol-Sulfuric Acid Method |
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