| 冰醋酸对于测定植物材料中超氧阴离子含量的灵敏度的影响 |
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| 引用本文： | 陆巍,许晓明,张荣铣,戴新宾. 冰醋酸对于测定植物材料中超氧阴离子含量的灵敏度的影响[J]. 南京师大学报(自然科学版), 2004, 27(1): 82-84 |
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| 作者姓名： | 陆巍 许晓明 张荣铣 戴新宾 |
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| 作者单位： | 南京农业大学生命科学学院,210095,南京;南京农业大学生命科学学院,210095,南京;南京农业大学生命科学学院,210095,南京;南京农业大学生命科学学院,210095,南京 |
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| 基金项目： | 国家重点基础研究发展规划项目 (G19980 10 10 0 )资助 |
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| 摘 要： | 利用羟胺氧化法测定超氧阴离子 ,在偶氮反应试剂中加入冰醋酸能够提高检测灵敏度 5 0 % .在植物材料的O2 - · 的提取过程中同时加入EDTA和羟胺 ,抑制SOD活性并及时“固定”O2 - · ,这样所测得的O2 - · 含量更能反映体内的实际情况 .同时发现提取上清液照光不产生O2 - · ,间接验证了O2 - · 来源于光合膜系统 .
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| 关 键 词： | 植物材料 超氧阴离子 含量 灵敏度 光合膜 |
| 文章编号： | 1001-4616(2004)01-0082-03 |
| 修稿时间： | 2003-09-11 |
Effece of Adding Acetic Acid on Improvement of Determination of Superoxide Anion Content in Plants |
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| Lu Wei,Xu Xiaoming,Zhang Rongxian,Dai Xinbin. Effece of Adding Acetic Acid on Improvement of Determination of Superoxide Anion Content in Plants[J]. Journal of Nanjing Normal University(Natural Science Edition), 2004, 27(1): 82-84 |
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| Authors: | Lu Wei Xu Xiaoming Zhang Rongxian Dai Xinbin |
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| Abstract: | Method for superoxide anion content determination through hydroxylammonium oxidation was greatly improved by adding acetic acid to the reagents of nitroazo reaction, which led to obvious increase in sensitivity by 50%. When superoxide was extracted from plant, EDTA and hydroxylammonium were added simultaneously to inhibit SOD activity and to fix superoxide immediately. The result thus obtained reflected more precisely the actual situation in plant. Superoxide anion was not produced by irradiation of extract supernatant, which suggests that it be originated from photosynthetic membrane. |
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| Keywords: | plant superoxide anion content sensitivity photosynthetic membrane |
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