| 浸矿细菌胞外聚合层中多糖的提取 |
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| 引用本文： | 富瑶,杨洪英,佟琳琳,张长征.浸矿细菌胞外聚合层中多糖的提取[J].东北大学学报(自然科学版),2010,31(2):228-231. |
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| 作者姓名： | 富瑶 杨洪英 佟琳琳 张长征 |
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| 作者单位： | 东北大学材料与冶金学院,辽宁,沈阳,110004;东北大学材料与冶金学院,辽宁,沈阳,110004;东北大学材料与冶金学院,辽宁,沈阳,110004;东北大学材料与冶金学院,辽宁,沈阳,110004 |
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| 基金项目： | 国家自然科学基金资助项目(50674029,50874030);;国家高技术研究发展计划项目(2006AA06Z127);;教育部高等学校博士学科点专项科研基金资助项目(20060145015);;中央高校基本科研业务费资助项目(N090602010) |
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| 摘 要： | 采用EDTA和H2SO4两种溶液作为提取剂,通过离心收集菌体、提取剂浸提多糖、离心分离多糖和过滤去除菌体4个步骤,提取浸矿细菌胞外聚合层中的多糖,并用苯酚-硫酸法测定提取出的多糖的含量.实验结果表明:质量分数相同时,提取剂EDTA的提取效果优于H2SO4;质量分数为3%的EDTA溶液为实验中的最优提取剂,提取的多糖质量浓度可达17.0 mg.L-1.整个提取过程具有使用试剂简单、操作简便、干扰因素少的特点.
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| 关 键 词： | 胞外聚合层 生物冶金 浸矿细菌 多糖 |
Polysaccharide Extraction from Extracellular Polymeric Substance via Bioleaching |
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| FU Yao,YANG Hong-ying,TONG Lin-lin,ZHANG Chang-zheng.Polysaccharide Extraction from Extracellular Polymeric Substance via Bioleaching[J].Journal of Northeastern University(Natural Science),2010,31(2):228-231. |
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| Authors: | FU Yao YANG Hong-ying TONG Lin-lin ZHANG Chang-zheng |
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| Institution: | School of Materials & Metallurgy;Northeastern University;Shenyang 110004;China. |
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| Abstract: | With the EDTA(ethylene diamine tetraacetic acid) and H2SO4(sulfuric acid)solutions both used as the extractants to extract the polysaccharide from EPS(extracellular polymeric substance) via bioleaching,the extracting process includes four steps,i.e.,collecting thallus by centrifugation,leaching polysaccharide with extractants,separating polysaccharide by centrifugation and filtering out thallus.The extracted polysaccharide content was measured by phenol-sulfuric acid.The results showed that the extractant E... |
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| Keywords: | EPS (extracellular polymeric substance) biological metallurgy bioleaching polysaccharide |
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