| 聚乙二醇改性壳聚糖固定化L-天门冬酰胺酶反应性质研究 |
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| 引用本文： | 霍红光,方波,江体乾.聚乙二醇改性壳聚糖固定化L-天门冬酰胺酶反应性质研究[J].华东理工大学学报(自然科学版),2005,31(3):277-280. |
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| 作者姓名： | 霍红光 方波 江体乾 |
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| 作者单位： | 华东理工大学化学工程研究所,上海,200237 |
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| 摘 要： | 研究了聚乙二醇改性壳聚糖(PEG-CS)固定化L-天门冬酰胺酶催化缓冲液中天冬酰胺的反应过程,考察了底物浓度、给酶量、温度、搅拌速率等条件对固定化酶在缓冲液中间歇催化反应过程的影响;以及底物浓度、流量、温度、床层高度等因素对固定化酶在缓冲液中连续催化反应过程的影响。实验结果表明:在适当的反应条件下,聚乙二醇改性壳聚糖固定化L-天门冬酰胺酶可以很好地水解缓冲液中的天冬酰胺。
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| 关 键 词： | 聚乙二醇改性壳聚糖 固定化L-天门冬酰胺酶 酶催化反应 |
| 文章编号： | 1006-3080(2005)03-0277-04 |
| 修稿时间： | 2004年6月6日 |
Reaction Properties of L-Asparaginase Immobilized on Chitosan Modified with Polyethylene Glycol |
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| HUO Hong-guang,FANG Bo,JIANG Ti-qian.Reaction Properties of L-Asparaginase Immobilized on Chitosan Modified with Polyethylene Glycol[J].Journal of East China University of Science and Technology,2005,31(3):277-280. |
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| Authors: | HUO Hong-guang FANG Bo JIANG Ti-qian |
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| Institution: | HUO Hong-guang,FANG Bo~*,JIANG Ti-qian |
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| Abstract: | Reaction properties of L-asparaginase immobilized on chitosan modified with polyethylene glycol were studied in this paper. The effects of substrate concentration, quantity of immobilized L-asparaginase, temperature, rotation rate on the batch reaction were investigated. Effects of substrate concentration, volume rate, temperature, height of enzyme column on the continuous reaction were investigated. The results show that under the proper conditions the immobilized L-asparaginase has good properties for hydrating asparagine from buffer. |
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| Keywords: | polyethylene glycol-chitosan immobilized L-asparaginase enzyme catalysis reaction |
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