大鼠再生肝8种细胞的嘌呤核苷酸代谢基因转录谱预示的代谢活动

为了解大鼠肝再生中肝细胞、胆管上皮细胞、卵圆细胞、星形细胞、窦内皮细胞、库普弗细胞、陷窝细胞、树突状细胞等8种肝脏细胞的嘌呤核苷酸代谢基因转录谱及预示的代谢活动,按张丽君[1]等方法分离大鼠部分肝切除后10个恢复时间点大鼠再生肝的上述8种细胞,用RatGenome2302.0芯片等检测嘌呤核苷酸代谢基因在上述细胞中表达变化,用Excel等软件及生物信息学和系统生物学等方法分析它们的表达模式、预示的生理活动等.结果表明,85个嘌呤核苷酸代谢基因在大鼠肝再生中发生了有意义表达变化,8种细胞的相应基因数为40,43,30,42,22,26,36和48.上调、下调、上/下调的基因个数为49、11、31,相应细胞的基因数为27、20和1,31、4和1,15、7和3,12、10和0,23、15和3,19、7和2,39、3和1,33、6和0.其中,催化DNA合成的DNA聚合酶基因和催化RNA合成的RNA聚合酶基因在肝再生的多个时间点和多种细胞中表达增强,胆管上皮细胞和星形细胞的腺苷酸合成相关基因表达增强.肝细胞、卵圆细胞和星形细胞的核苷酸分解相关基因、肝细胞、星形细胞和树突状细胞的嘌呤核苷分解相关基因表达减弱.预示大鼠肝再生与嘌呤核苷酸代谢密切相关.

Transcript Profiles of Purine Nucleotide Metabolism-Associated Genes Uncovers the Metabolism Activities in Rat Eight Kinds of Regenerating Liver Cells

To study the transcript profiles of purine nucleotide metabolism-associated genes in 8 kinds of regenerating liver cells and the metabolism activities they predicted,rat 8 liver cell types including hepatocytes,bile duct epithelial cells,oval cells,stellate cells,sinus endothelial cells,Kupffer cells,pit cells and dendritic cells were separated and purified respectively from 10 recovery time points in rat liver regeneration after rat partial hepatectomy following Zhang Lijun et al methods[1]. The Rat Genome 230 2.0 high-throughput array were used for detecting gene expression changes in the above 8 liver cell types. With H-cluster software and bioinformatics and systems biology methods,their expression patterns and the predicted physical activity were analyzed. The results showed that a total of 85 purine nucleotide metabolism-associated genes yielded the meaningful expression changes in liver regeneration,the corresponding gene numbers in 8 liver cell types were 40,43,30,42,22,26,36 and 48. The number of up-,down-and up-/down-regulated genes are 49,11,31,the number of corresponding genes in 8 cell types are 27,20 and 1,31,4 and 1,15,7 and 3,12,10 and 0,23,15 and 3,19,7 and 2,39,3 and 1,33,6 and 0. Among them,the expression of DNA polymerase genes and RNA polymerase gene increased in multiple time points and a variety of cells during rat liver regeneration. The expression of AMP synthesis-related genes of bile duct epithelial cells and hepatic stellate cells increased. The expression of genes participating in nucleotide decomposing to nucleosides decreased in hepatocytes,oval cells and hepatic stellate cells,as well as purine nucleosidet to purine decreased in hepatocytes,hepatic stellate cells and dendritic cells. The gene transcript profiles predicted that the rat liver regeneration is closely associated with the purine nucleotide metabolism.