8-羟基喹啉和对苯二酚诱导蚕豆根尖细胞异常性的比较研究

采用8-羟基喹啉与对苯二酚处理蚕豆根尖,根尖经恢复培养后,以孚尔根染色法制片观察.同时以低温处理的相同材料为对照.经2种药物不同浓度处理后观察其根尖细胞的微核率,以及它们对有丝分裂指数的影响,同时还观察了它们对染色体畸变的效应.得出以下结果:(1)2种诱变剂均能诱发高频率的微核细胞;8-HQ还可以产生较高频率的双核细胞.(2)HQ在2个不同处理浓度均可明显提高有丝分裂指数,且可有效地积累中期分裂相;而8-HQ对有丝分裂指数的提高影响不明显,且在0.004 mol.L-1浓度时对有丝分裂指数具有显著的抑制效应.(3)2种诱变剂均能导致染色体畸变及其他核异常.本研究结果表明8-HQ和HQ均是细胞板有效的抑制剂和诱发染色体数目和结构变异较强的诱变剂.

A Comparative Study of Abnormalities of Cells in Roots of Vicia faba Induced by 8-Hydroxylquinoline and Hydroquinone

Root tip of vicia faba,first treated with 8-HQ and HQ separately and then cultivated for recovery,are sliced and observed according to Feulgen staining method with material treated at low temperature as the negative control.The micronucleus rate of cells in the Roots tips treated with the two chemicals each of different densities and the effect of the chemicals on mitosis index and chromosome aberration are examined.After comparing with the control sample the following conclusions are drawn:(1) Both mutagens can induce high frequency micronucleus;8-HQ can also produce binuclear cells at a relatively high frequency.(2) HQ of both different densities can obviously increase mitosis index and effectively accumulate mid-term split phase while 8-HQ exerts litter effect on the increase of mitosis index but shows remarkable restraining effect at 0.004 mol·L-1.(3) Both mutagens can induce chromosome aberration and nuclear aberration of other kinds.The results of this study show that both 8-HQ and HQ are effective cell board restraining agents and strong mutagens inducing aberration in the number and structure of chromosomes.