| 糙皮侧耳831原生质体的分离和再生 |
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| 引用本文： | 何方淑,牛若芳.糙皮侧耳831原生质体的分离和再生[J].河南师范大学学报(自然科学版),1990(2):64-65. |
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| 作者姓名： | 何方淑 牛若芳 |
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| 作者单位： | 河南师范大学生物系，河南师范大学生物系，河南师范大学生物系，河南师范大学生物系 |
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| 摘 要： | 本试验利用蜗牛酶和溶壁酶处理糙皮侧耳幼嫩菌丝,成功地分离出原生质体,原生质体数量为6.0×10~5～1.07×10~7个/ml;再生频率为1.38%,再生菌株出菇正常,生物学效率多在100%以上。
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| 关 键 词： | 糙皮侧耳 原生质体 再生菌株 |
IOSLATION AND RGENERATION OF PROTOPLASTS OF PLEUROTUS OSTREATUS 831 |
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| He Fangshu,Niu Ruofang,Shan Yingfang,Li zhongyi.IOSLATION AND RGENERATION OF PROTOPLASTS OF PLEUROTUS OSTREATUS 831[J].Journal of Henan Normal University(Natural Science),1990(2):64-65. |
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| Authors: | He Fangshu Niu Ruofang Shan Yingfang Li zhongyi |
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| Institution: | He Fangshu;Niu Ruofang;Shan Yingfang;Li zhongyi Department of Biology |
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| Abstract: | The isolation and regeneration of yong mycelial protolasts from pleurotus ostreatus 831 using 2% snailase and0.7% lywallzyme were studied.2 ml mixed enzyme was added to 100mg mycelia(wet weight),and pH5.8 bufferfor enzymolysis at 30℃ for 2 hrs,and 6.0×10~5—1.07×10~7 protoplasts/ml were achieved.The regeneration fre-quency of protoplasts by using RM soft agar medium was 1.36%.The strains of regeneration fruiting experimentwere studied,and boilogical effciency above 100% acheved. |
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| Keywords: | Pleurotus ostreatus protoplasts regeneration strain |
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