槐属二氢黄酮G促进L6细胞内GLUT4的表达和转运

利用细胞葡萄糖摄取检测试剂盒检测苦参中槐属二氢黄酮G(Sophoraflavanone G，SFG) 对L6细胞葡萄糖摄取的影响，发现SFG能够增加大鼠成肌细胞 (L6) 的葡萄糖摄取；之后，使用Western blot检测发现SFG对L6细胞内GLUT4的表达有显著的促进作用；同时，在可稳定表达IRAP-mOrange荧光蛋白的L6细胞内，使用激光共聚焦显微镜监测SFG作用下葡萄糖转位蛋白4 (glucose transporter 4，GLUT4) 的转位，发现SFG对GLUT4的转位有显著的促进作用，而且SFG对GLUT4转位的促进呈浓度依赖性；另外，免疫荧光实验结果也显示SFG增强L6细胞中GLUT4与细胞膜的融合.这些结果显示利用SFG处理L6细胞后，L6细胞内GLUT4的表达、转运及与细胞膜的融合继而促进葡萄糖的摄取显著增强，说明SFG可能具备开发成一种新的降血糖药物的潜力.

Sophoraflavanone G promote the expression and translocation of GLUT4 in L6 cells

In this study，the effects of Sophoraflavanone G (SFG) on glucose uptake were detected in L6 cells by cell glucose uptake assay kit. We found that SFG increased glucose uptake in L6 cells. Western blotting was used to detect the effects of SFG on GLUT4 expression level， and it was found that SFG significantly promoted GLUT4 expression in L6 cells. Meanwhile， the translocation of GLUT4 also was monitored. We found that SFG significantly promoted GLUT4 translocation， and the effects of SFG on the translocation of GLUT4 was concentration dependent in L6 cells. In addition， immunofluorescence test results also showed that SFG enhanced the fusion of GLUT4 and cell membrane in L6 cells.These results showed that SFG promoted glucose uptake by improving GLUT4 expression， translocation and fusion with cell membrane in L6 cell and SFG might have the potential to develop a new hypoglycemic drug.