双酚A高效降解菌的筛选与降解特性

从受双酚A严重污染的土壤中获得菌种, 分离纯化筛选出优势菌种, 经驯化培养增强其降解能力. 一株最高效的菌种经形态鉴定其为短杆菌. 通过摇瓶实验考察了生长条件对该菌株生长和底物降解的影响, 在双酚A浓度为50.18 mg/L时得出其最适合的生长条件为： pH=4, NH₄Cl=4.12 mg/L, KH₂PO₄ =0.946 4 mg/L, 降解双酚A饱和溶液的最佳接种量为5%, 8 d后降解率可达到71.79%.

Screen of Bacteria High-effectively Degrading Bisphenol A and Its Degradation Characteristics

Bisphenol A(BPA) is an environmental hormone polluting widely.The bacterial strains degrading BPA were isolated from its deeply polluted soil.By acclimation,their capacities for the degradation of BPA were enhanced.The strain that had the best degradation capacity was selected.It was identified as short bacillus according to its morphological character.By tests in shaking flasks,the effects of the conditions of the growth were studied,and the optimum conditions of the growth of the strain were: pH=4,NH_4Cl=4.12(mg/L),KH_2PO_4=0.946 4 mg/L,the best bacterial addition amount was 5%,the degraded ratio of BPA reached 71.79% after degradation for 8 days at concentration of 50 mg/L in solution.