安石榴甙分子印迹聚合物微球的制备及识别性能

以安石榴甙为模板分子，丙烯酰胺为功能单体，乙二醇二甲基丙烯酸酯为交联剂，偶氮二异丁腈(AIBN)为引发剂，乙腈为溶剂，使用沉淀聚合方法，制备分子印迹聚合物，得到纳米级微球。等温吸附实验研究表明，印迹聚合物与空白聚合物相比，对目标分子具有更好的吸附性能，在研究浓度范围内，印迹聚合物对目标分子的最大吸附量为32.6μmol/g;Scatchard分析表明，印迹聚合物具有两种不同性能的结合位点，空白聚合物有一种结合位点，印迹聚合物的最大表观结合量为243μmol/g，空白聚合物的最大表观结合量为25.3μmol/g；底物选择实验表明分子印迹聚合物对安石榴甙具有更高的选择性能。

Preparation of molecularly imprinted microspheres and their recognition properties for punicalagin

Molecularly imprinted polymers(MIPs) for recognition of punicalagin were prepared by precipitation polymerization using punicalagin as template,acrylamide(AM) as the functional monomer,ethylene dimethacrylate(EDMA) as the cross-linker,azoisobutyronitrile(AIBN) as the initiator and acetonitrile as the solvent.The MIPs prepared formed spherical beads with nanometer sizes.The results of equilibrium adsorption experiments showed that the MIPs had a high affinity to the template in comparison with the blank polymers(BPs),and the max adsorption of MIPs was 32.6μmol/g under the studied concentration;Scatchard analysis showed that two kinds of binding sites were formed in the MIPs and one kind of binding site in the Bps,the apparent maximum binding capacity was 243μmol/g,whilst for the Bps,the apparent maximum binding capacity were 25.3μmol/g;the selective binding experiment showed that the MIPs had higher selectivity and affinity.